Holly Huse
Investigator, The Lundquist Institute
Director of Microbiology, Harbor-UCLA Medical Center
Contact
Email
Evaluation of antimicrobial susceptibility testing methods
Research Description
One of the most important tests performed by the clinical microbiology laboratory is antimicrobial susceptibility testing (AST), which provides guidance to physicians on appropriate antimicrobial therapy for their patients. Therefore, AST methods must be accurate, reproducible, and associated with clinical outcomes. My research focuses on studying the accuracy and reproducibility of AST methods for less commonly isolated organisms, such as those within the Burkholderia cepacia complex.Theme Groups
Research Interests
Education
- PhD, 2013, Microbiology, University of Texas at Austin
Recent and/or Significant Publications
Buchan BW, Windham S, Balada-Llasat JM, Leber A, Harrington A, Relich R, Murphy C, Dien Bard J, Naccache S, Ronen S, Hopp A, Mahmutoglu D, Faron ML, Ledeboer NA, Carroll A, Stone H, Akerele O, Everhart K, Bonwit A, Kwong C, Buckner R, Warren D, Fowler R, Chandrasekaran S, Huse H, Campeau S, Humphries R, Graue C, Huang A. Practical Comparison of the BioFire FilmArray Pneumonia Panel to Routine Diagnostic Methods and Potential Impact on Antimicrobial Stewardship in Adult Hospitalized Patients with Lower Respiratory Tract Infections. J Clin Microbiol. 2020 Jun 24;58(7):e00135-20. doi: 10.1128/JCM.00135-20. PMID: 32350045; PMCID: PMC7315039.
Huse HK, Miller SA, Chandrasekaran S, Hindler JA, Lawhon SD, Bemis DA, Westblade LF, Humphries RM. Evaluation of Oxacillin and Cefoxitin Disk Diffusion and MIC Breakpoints Established by the Clinical and Laboratory Standards Institute for Detection of mecA-Mediated Oxacillin Resistance in Staphylococcus schleiferi. J Clin Microbiol. 2018 Jan 24;56(2):e01653-17. doi: 10.1128/JCM.01653-17. PMID: 29187565; PMCID: PMC5786728.
Gallagher LA, Ramage E, Weiss EJ, Radey M, Hayden HS, Held KG, Huse HK, Zurawski DV, Brittnacher MJ, Manoil C. Resources for Genetic and Genomic Analysis of Emerging Pathogen Acinetobacter baumannii. J Bacteriol. 2015 Jun 15;197(12):2027-35. doi: 10.1128/JB.00131-15. Epub 2015 Apr 6. PMID: 25845845; PMCID: PMC4438207.
Huse HK, Kwon T, Zlosnik JE, Speert DP, Marcotte EM, Whiteley M. Parallel evolution in Pseudomonas aeruginosa over 39,000 generations in vivo. mBio. 2010 Sep 21;1(4):e00199-10. doi: 10.1128/mBio.00199-10. PMID: 20856824; PMCID: PMC2939680.
Cornforth DM, Dees JL, Ibberson CB, Huse HK, Mathiesen IH, Kirketerp-Møller K, Wolcott RD, Rumbaugh KP, Bjarnsholt T, Whiteley M. Pseudomonas aeruginosa transcriptome during human infection. Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5125-E5134. doi: 10.1073/pnas.1717525115. Epub 2018 May 14. PMID: 29760087; PMCID: PMC5984494.